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ヒト唾液ペプチドの抗菌機能を活用したヘルスケア製品の開発
https://niit.repo.nii.ac.jp/records/196
https://niit.repo.nii.ac.jp/records/19642737339-4315-4317-81bf-89575a1b8989
名前 / ファイル | ライセンス | アクション |
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15_9-14.pdf (4.8 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2011-03-02 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | ヒト唾液ペプチドの抗菌機能を活用したヘルスケア製品の開発 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A Study on Application of Human Salivary Peptides with Antimicrobial Activities to Healthcare Products | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | human saliva | |||||
キーワード | ||||||
主題 | histatin 3 | |||||
キーワード | ||||||
主題 | short proteolytic peptides | |||||
キーワード | ||||||
主題 | Porphyromonas gingivalis | |||||
キーワード | ||||||
主題 | antimicrobial peptide | |||||
キーワード | ||||||
主題 | membrane depolarization | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
大滝, 俊樹
× 大滝, 俊樹× 高橋, 信輝× 武井, 教展× 谷口, 正之× 斎藤, 英一 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Human salivary histidine-rich polypeptides, histatin 1 (38 ammo acids) and histatin 3 (32 aa) are major antimicrobial peptides harboring potent fungicidal activity against Candida albicans. This study was undertaken to examine the activity of four short peptide fragments, histatins 5 (24 aa), 8 (12 aa), 9 (14 aa), and 11 (8 aa), derived from histatin 3 against Porphyromonas gingivalis (penodontal bacteria). By determining the concentration of ATP synthesized by microorganism, histatins 5, 8, 9, and 11 were found to inhibit the growth of P. gingivalis with 50 % inhibitory concentrations (IC_50s) of 33.4, >800, 9.7, and 294.1 μM, respectively. Cytoplasmic membrane depolarization of P. gingivalis by the peptides was elucidated using the membrane-potential-sensitive dye, diSC_3-5. All of the fragments of histatin 3 caused membrane depolarization of 10.4 - 43.9 % at 33.4μM in a good's buffer (5 mM HEPES, pH 7.2, 50 mM glucose) and of 3.5 - 25 % in physiological condition (4 mM NaH_2PO_4/Na_2HP0_4, pH 7.0, 30mM NaCl), respectively. Taken together, it can be concluded that even shorter peptide fragments derived from human histatin 3, show potent antibactencidal activity against P. gingivalis. Thereby, antimicrobial peptides reported here appear to be excellent candidates for oral healthcare products. | |||||
書誌情報 |
新潟工科大学研究紀要 巻 15, p. 9-14, 発行日 2010-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1342-792X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10590360 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 新潟工科大学 |