We have studied the method of measuring and analyzing the electrical potential distribution in the trunk of a tree in order to estimate the location and the degree of the damage caused by nematode. When a branch of the tree was cut, its location was identified by the potential distribution map on the section of the tree analyzed by the proposed method. Moreover, the potential distribution on the section of the tree was compared with that on the section of a phantom (agar). Both distribution have shown the high correlation factor, and we can say that the method can be used to estimate the damage in the trunk of a tree.