{"created":"2023-05-15T08:55:28.953324+00:00","id":214,"links":{},"metadata":{"_buckets":{"deposit":"376bea54-4d74-4ebb-9a24-f3dcdf389afe"},"_deposit":{"created_by":4,"id":"214","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"214"},"status":"published"},"_oai":{"id":"oai:niit.repo.nii.ac.jp:00000214","sets":["6:7:24"]},"author_link":["884","885","886","887","888","889","878","879","880","881","882","883"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"34","bibliographicPageStart":"29","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"新潟工科大学研究紀要"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Human saliva contains histains (1 and 3) and their short proteolytic fragments. To study the antifungal mechanism and action against cell membrane of Candida albicans NBRC 1385, we prepared synthetic histatins 5(24 amino acids), 8(12 a.a.), 9(14 a.a.) and 11(8 a.a.). By cell viability assay, half maximal inhibitory concentration (IC50) of histains 5, 8, 9, and 11 was elucidated, respectively, to be 136.7, 251.7, 109.6, and 267.2 μM. When 20 μM melittin was employed as the 100 % control, the percentage of membrane depolarization caused by 20 μM histatins (5, 8, 9, and 11) was determined, respectively, to be 79.9, 16.8, 58.5, and 48.4 % with the membrane potential-sensitive dye diSC_3-5. Using 200 μM melittin as the 100 % control, the percentage of calcein leakage from C. albicans by 200 μM of above histatins was estimated, respectively, to be 65.1, 20.2, 42.8, and 13.2 %. Taken together it may be suggested that histatins attach to the cell membrane of C. albicans via electrostatic interactions. On the membrane surface, the histatins may not only form the pores by placing their hydrophobic part in contact with the hydrophobic core of membrane but also lead to the collapse of the membrane. Based on the ideas, it can be proposed that the interaction between histatins and phospholipid of cell membrane mediates C. albicans-killing process.","subitem_description_type":"Abstract"}]},"item_2_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟工科大学"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1342-792X","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10590360","subitem_source_identifier_type":"NCID"}]},"item_2_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大滝, 俊樹"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"笠原, 仁"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"武井, 教展"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"谷口, 正之"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"加藤, 哲男"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"斎藤, 英一"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-25"}],"displaytype":"detail","filename":"17_29-34.pdf","filesize":[{"value":"4.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"17_29-34.pdf","url":"https://niit.repo.nii.ac.jp/record/214/files/17_29-34.pdf"},"version_id":"176582c8-b3f6-4399-abdc-c587993d5229"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Human saliva"},{"subitem_subject":"antimicrobial peptide"},{"subitem_subject":"proteolysis"},{"subitem_subject":"short peptides"},{"subitem_subject":"histatin 3"},{"subitem_subject":"candida albicans"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヒト唾液短鎖ヒスタチンの口内炎菌（Candida albicans NBRC 1385株）の細胞膜に対する作動性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト唾液短鎖ヒスタチンの口内炎菌（Candida albicans NBRC 1385株）の細胞膜に対する作動性","subitem_title_language":"ja"},{"subitem_title":"Action of Human Salivary Short Histatins Against The Cell Membrane of Candida Albicans NBRC 1385","subitem_title_language":"en"}]},"item_type_id":"2","owner":"4","path":["24"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-02-05"},"publish_date":"2013-02-05","publish_status":"0","recid":"214","relation_version_is_last":true,"title":["ヒト唾液短鎖ヒスタチンの口内炎菌（Candida albicans NBRC 1385株）の細胞膜に対する作動性"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-06-20T05:55:52.473056+00:00"}