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水晶振動子型バイオセンサーによる生体分子間相互作用の研究
https://niit.repo.nii.ac.jp/records/171
https://niit.repo.nii.ac.jp/records/171bb3caf04-3467-4a24-981f-a1e0514a8e22
名前 / ファイル | ライセンス | アクション |
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13_19-24.pdf (850.9 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-11-08 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 水晶振動子型バイオセンサーによる生体分子間相互作用の研究 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A Study on the Biomacromolecular Interactions by Quartz-Crystal Microbalance Biosensor | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | Bowman-Birk type serine protease inhibitor | |||||
キーワード | ||||||
主題 | Chymotrypsin | |||||
キーワード | ||||||
主題 | Dissociation constant | |||||
キーワード | ||||||
主題 | Quartz crystal microbalance biosensor | |||||
キーワード | ||||||
主題 | Soybean trypsin inhibitor | |||||
キーワード | ||||||
主題 | Trypsin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
斎藤, 英一
× 斎藤, 英一× 笠原, 仁× 串宮, 範彦× 下村, 雅人 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Two serine protease inhibitors from soybeans, Kunitz type soybean trypsin inhibitor (STI) and Bowman-Birk inhibitor (BBI) were immobilized via an alkanethiol onto a gold surface on a highly sensitive 21 MHz AT-cut quartz-crystal microbalance (QCM) chip. The QCM exhibited a significant decrease in frequency responding to serine protease addition. The dissociation constants (Kd) for the interactions of STI-bovine pancreatic trypsin, BBI-bovine pancreatic trypsin, and BBI-bovine pancreatic chymotrypsin were determined, respectively, to be 47.0 nM, 83.6 nM, and 52.9 nM. The data suggest that the inhibitors were immobilized on the gold surface of QCM in a possible orientation to react with corresponding serine proteases. Based on the observations, it may be concluded that combining protease inhibitor and protease recognition events with an appropriate QCM transducer can yield sensor devices highly suitable for detection of proteases in food, clinical and biodefense areas. | |||||
書誌情報 |
新潟工科大学研究紀要 巻 13, p. 19-24, 発行日 2008-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1342-792X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10590360 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 新潟工科大学 |